RXLR and CRN Effectors from the Sunflower Downy Mildew Pathogen Plasmopara halstedii Induce Hypersensitive-Like Responses in Resistant Sunflower Lines.
Identifieur interne : 000276 ( Main/Exploration ); précédent : 000275; suivant : 000277RXLR and CRN Effectors from the Sunflower Downy Mildew Pathogen Plasmopara halstedii Induce Hypersensitive-Like Responses in Resistant Sunflower Lines.
Auteurs : Quentin Gascuel [France] ; Luis Buendia [France] ; Yann Pecrix [France] ; Nicolas Blanchet [France] ; Stéphane Mu Os [France] ; Felicity Vear [France] ; Laurence Godiard [France]Source :
- Frontiers in plant science [ 1664-462X ] ; 2016.
Abstract
Plasmopara halstedii is an obligate biotrophic oomycete causing downy mildew disease on sunflower, Helianthus annuus, an economically important oil crop. Severe symptoms of the disease (e.g., plant dwarfism, leaf bleaching, sporulation and production of infertile flower) strongly impair seed yield. Pl resistance genes conferring resistance to specific P. halstedii pathotypes were located on sunflower genetic map but yet not cloned. They are present in cultivated lines to protect them against downy mildew disease. Among the 16 different P. halstedii pathotypes recorded in France, pathotype 710 is frequently found, and therefore continuously controlled in sunflower by different Pl genes. High-throughput sequencing of cDNA from P. halstedii led us to identify potential effectors with the characteristic RXLR or CRN motifs described in other oomycetes. Expression of six P. halstedii putative effectors, five RXLR and one CRN, was analyzed by qRT-PCR in pathogen spores and in the pathogen infecting sunflower leaves and selected for functional analyses. We developed a new method for transient expression in sunflower plant leaves and showed for the first time subcellular localization of P. halstedii effectors fused to a fluorescent protein in sunflower leaf cells. Overexpression of the CRN and of 3 RXLR effectors induced hypersensitive-like cell death reactions in some sunflower near-isogenic lines resistant to pathotype 710 and not in susceptible corresponding lines, suggesting they could be involved in Pl loci-mediated resistances.
DOI: 10.3389/fpls.2016.01887
PubMed: 28066456
PubMed Central: PMC5165252
Affiliations:
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Microorganismes, INRA, CNRS, Université de Toulouse Castanet Tolosan</wicri:regionArea><wicri:noRegion>Université de Toulouse Castanet Tolosan</wicri:noRegion><wicri:noRegion>Université de Toulouse Castanet Tolosan</wicri:noRegion></affiliation></author><author><name sortKey="Buendia, Luis" sort="Buendia, Luis" uniqKey="Buendia L" first="Luis" last="Buendia">Luis Buendia</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire des Interactions Plantes Microorganismes, INRA, CNRS, Université de Toulouse Castanet Tolosan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire des Interactions Plantes Microorganismes, INRA, CNRS, Université de Toulouse Castanet Tolosan</wicri:regionArea><wicri:noRegion>Université de Toulouse Castanet Tolosan</wicri:noRegion><wicri:noRegion>Université de Toulouse Castanet Tolosan</wicri:noRegion></affiliation></author><author><name sortKey="Pecrix, Yann" sort="Pecrix, Yann" uniqKey="Pecrix Y" first="Yann" last="Pecrix">Yann Pecrix</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire des Interactions Plantes Microorganismes, INRA, CNRS, Université de Toulouse Castanet Tolosan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire des Interactions Plantes Microorganismes, INRA, CNRS, Université de Toulouse Castanet Tolosan</wicri:regionArea><wicri:noRegion>Université de Toulouse Castanet Tolosan</wicri:noRegion><wicri:noRegion>Université de Toulouse Castanet Tolosan</wicri:noRegion></affiliation></author><author><name sortKey="Blanchet, Nicolas" sort="Blanchet, Nicolas" uniqKey="Blanchet N" first="Nicolas" last="Blanchet">Nicolas Blanchet</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire des Interactions Plantes Microorganismes, INRA, CNRS, Université de Toulouse Castanet Tolosan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire des Interactions Plantes Microorganismes, INRA, CNRS, Université de Toulouse Castanet Tolosan</wicri:regionArea><wicri:noRegion>Université de Toulouse Castanet Tolosan</wicri:noRegion><wicri:noRegion>Université de Toulouse Castanet Tolosan</wicri:noRegion></affiliation></author><author><name sortKey="Mu Os, Stephane" sort="Mu Os, Stephane" uniqKey="Mu Os S" first="Stéphane" last="Mu Os">Stéphane Mu Os</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire des Interactions Plantes Microorganismes, INRA, CNRS, Université de Toulouse Castanet Tolosan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire des Interactions Plantes Microorganismes, INRA, CNRS, Université de Toulouse Castanet Tolosan</wicri:regionArea><wicri:noRegion>Université de Toulouse Castanet Tolosan</wicri:noRegion><wicri:noRegion>Université de Toulouse Castanet Tolosan</wicri:noRegion></affiliation></author><author><name sortKey="Vear, Felicity" sort="Vear, Felicity" uniqKey="Vear F" first="Felicity" last="Vear">Felicity Vear</name><affiliation wicri:level="1"><nlm:affiliation>INRA, UMR1095 Clermont-Ferrand, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, UMR1095 Clermont-Ferrand</wicri:regionArea><wicri:noRegion>UMR1095 Clermont-Ferrand</wicri:noRegion><wicri:noRegion>UMR1095 Clermont-Ferrand</wicri:noRegion></affiliation></author><author><name sortKey="Godiard, Laurence" sort="Godiard, Laurence" uniqKey="Godiard L" first="Laurence" last="Godiard">Laurence Godiard</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire des Interactions Plantes Microorganismes, INRA, CNRS, Université de Toulouse Castanet Tolosan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire des Interactions Plantes Microorganismes, INRA, CNRS, Université de Toulouse Castanet Tolosan</wicri:regionArea><wicri:noRegion>Université de Toulouse Castanet Tolosan</wicri:noRegion><wicri:noRegion>Université de Toulouse Castanet Tolosan</wicri:noRegion></affiliation></author></analytic><series><title level="j">Frontiers in plant science</title><idno type="ISSN">1664-462X</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><i>Plasmopara halstedii</i> is an obligate biotrophic oomycete causing downy mildew disease on sunflower, <i>Helianthus annuus</i>, an economically important oil crop. Severe symptoms of the disease (e.g., plant dwarfism, leaf bleaching, sporulation and production of infertile flower) strongly impair seed yield. <i>Pl</i> resistance genes conferring resistance to specific <i>P. halstedii</i> pathotypes were located on sunflower genetic map but yet not cloned. They are present in cultivated lines to protect them against downy mildew disease. Among the 16 different <i>P. halstedii</i> pathotypes recorded in France, pathotype 710 is frequently found, and therefore continuously controlled in sunflower by different <i>Pl</i> genes. High-throughput sequencing of cDNA from <i>P. halstedii</i> led us to identify potential effectors with the characteristic RXLR or CRN motifs described in other oomycetes. Expression of six <i>P. halstedii</i> putative effectors, five RXLR and one CRN, was analyzed by qRT-PCR in pathogen spores and in the pathogen infecting sunflower leaves and selected for functional analyses. We developed a new method for transient expression in sunflower plant leaves and showed for the first time subcellular localization of <i>P. halstedii</i> effectors fused to a fluorescent protein in sunflower leaf cells. Overexpression of the CRN and of 3 RXLR effectors induced hypersensitive-like cell death reactions in some sunflower near-isogenic lines resistant to pathotype 710 and not in susceptible corresponding lines, suggesting they could be involved in <i>Pl</i> loci-mediated resistances.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">28066456</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>01</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-462X</ISSN><JournalIssue CitedMedium="Print"><Volume>7</Volume><PubDate><Year>2016</Year></PubDate></JournalIssue><Title>Frontiers in plant science</Title><ISOAbbreviation>Front Plant Sci</ISOAbbreviation></Journal><ArticleTitle>RXLR and CRN Effectors from the Sunflower Downy Mildew Pathogen <i>Plasmopara halstedii</i> Induce Hypersensitive-Like Responses in Resistant Sunflower Lines.</ArticleTitle><Pagination><MedlinePgn>1887</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fpls.2016.01887</ELocationID><Abstract><AbstractText><i>Plasmopara halstedii</i> is an obligate biotrophic oomycete causing downy mildew disease on sunflower, <i>Helianthus annuus</i>, an economically important oil crop. Severe symptoms of the disease (e.g., plant dwarfism, leaf bleaching, sporulation and production of infertile flower) strongly impair seed yield. <i>Pl</i> resistance genes conferring resistance to specific <i>P. halstedii</i> pathotypes were located on sunflower genetic map but yet not cloned. They are present in cultivated lines to protect them against downy mildew disease. Among the 16 different <i>P. halstedii</i> pathotypes recorded in France, pathotype 710 is frequently found, and therefore continuously controlled in sunflower by different <i>Pl</i> genes. High-throughput sequencing of cDNA from <i>P. halstedii</i> led us to identify potential effectors with the characteristic RXLR or CRN motifs described in other oomycetes. Expression of six <i>P. halstedii</i> putative effectors, five RXLR and one CRN, was analyzed by qRT-PCR in pathogen spores and in the pathogen infecting sunflower leaves and selected for functional analyses. We developed a new method for transient expression in sunflower plant leaves and showed for the first time subcellular localization of <i>P. halstedii</i> effectors fused to a fluorescent protein in sunflower leaf cells. Overexpression of the CRN and of 3 RXLR effectors induced hypersensitive-like cell death reactions in some sunflower near-isogenic lines resistant to pathotype 710 and not in susceptible corresponding lines, suggesting they could be involved in <i>Pl</i> loci-mediated resistances.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Gascuel</LastName><ForeName>Quentin</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>Laboratoire des Interactions Plantes Microorganismes, INRA, CNRS, Université de Toulouse Castanet Tolosan, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Buendia</LastName><ForeName>Luis</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Laboratoire des Interactions Plantes Microorganismes, INRA, CNRS, Université de Toulouse Castanet Tolosan, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pecrix</LastName><ForeName>Yann</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Laboratoire des Interactions Plantes Microorganismes, INRA, CNRS, Université de Toulouse Castanet Tolosan, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Blanchet</LastName><ForeName>Nicolas</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Laboratoire des Interactions Plantes Microorganismes, INRA, CNRS, Université de Toulouse Castanet Tolosan, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Muños</LastName><ForeName>Stéphane</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Laboratoire des Interactions Plantes Microorganismes, INRA, CNRS, Université de Toulouse Castanet Tolosan, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vear</LastName><ForeName>Felicity</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>INRA, UMR1095 Clermont-Ferrand, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Godiard</LastName><ForeName>Laurence</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Laboratoire des Interactions Plantes Microorganismes, INRA, CNRS, Université de Toulouse Castanet Tolosan, France.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>12</Month><Day>19</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Plant Sci</MedlineTA><NlmUniqueID>101568200</NlmUniqueID><ISSNLinking>1664-462X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Agrobacterium-mediated transient expression</Keyword><Keyword MajorTopicYN="N">Plasmopara halstedii</Keyword><Keyword MajorTopicYN="N">downy mildew</Keyword><Keyword MajorTopicYN="N">hypersensitive response</Keyword><Keyword MajorTopicYN="N">oomycete effectors</Keyword><Keyword MajorTopicYN="N">resistant sunflower</Keyword><Keyword MajorTopicYN="N">subcellular localization</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>09</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>11</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>1</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>1</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>1</Month><Day>10</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28066456</ArticleId><ArticleId 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IdType="pubmed">22143776</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>France</li></country></list><tree><country name="France"><noRegion><name sortKey="Gascuel, Quentin" sort="Gascuel, Quentin" uniqKey="Gascuel Q" first="Quentin" last="Gascuel">Quentin Gascuel</name></noRegion><name sortKey="Blanchet, Nicolas" sort="Blanchet, Nicolas" uniqKey="Blanchet N" first="Nicolas" last="Blanchet">Nicolas Blanchet</name><name sortKey="Buendia, Luis" sort="Buendia, Luis" uniqKey="Buendia L" first="Luis" last="Buendia">Luis Buendia</name><name sortKey="Godiard, Laurence" sort="Godiard, Laurence" uniqKey="Godiard L" first="Laurence" last="Godiard">Laurence Godiard</name><name sortKey="Mu Os, Stephane" sort="Mu Os, Stephane" uniqKey="Mu Os S" first="Stéphane" last="Mu Os">Stéphane Mu Os</name><name sortKey="Pecrix, Yann" sort="Pecrix, Yann" uniqKey="Pecrix Y" first="Yann" last="Pecrix">Yann Pecrix</name><name sortKey="Vear, Felicity" sort="Vear, Felicity" uniqKey="Vear F" first="Felicity" last="Vear">Felicity Vear</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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